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Cytotoxicity evaluation of large cyanobacterial strain set using selected human and murine in vitro cell models

SYSNO ASEP	0464589
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cytotoxicity evaluation of large cyanobacterial strain set using selected human and murine in vitro cell models
Author(s)	Hrouzek, Pavel (MBU-M)_ORCID Kapuscik, Alexandra (MBU-M) Vacek, J. (CZ) Voráčková, Kateřina (MBU-M) Paichlová, Jindřiška (MBU-M) Kosina, P. (CZ) Voloshko, L. (RU) Ventura, S. (IT) Kopecký, Jiří (MBU-M)_ORCID
Source Title	Ecotoxicology and Enviromental Safety. - : Elsevier - ISSN 0147-6513 Roč. 124, February (2016), s. 177-185
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	Cytotoxicity ; Cyanobacteria ; Cell lines
Subject RIV	EE - Microbiology, Virology
R&D Projects	GPP503/12/P614 GA ČR - Czech Science Foundation (CSF)
	GA14-18067S GA ČR - Czech Science Foundation (CSF)
	ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000369881000023
EID SCOPUS	84945367592
DOI	10.1016/j.ecoenv.2015.10.020
Annotation	The production of cytotoxic molecules interfering with mammalian cells is extensively reported in cyanobacteria. These compounds may have a use in pharmacological applications; however, their potential toxicity needs to be considered. We performed cytotoxicity tests of crude cyanobacterial extracts in six cell models in order to address the frequency of cyanobacterial cytotoxicity to human cells and the level of specificity to a particular cell line. A set of more than 100 cyanobacterial crude extracts isolated from soil habitats (mainly genera Nostoc and Tolypothrix) was tested by MTT test for in vitro toxicity on the hepatic and non-hepatic human cell lines HepG2 and HeLa, and three cell systems of rodent origin: Yac-1, Sp-2 and Balb/c 3T3 fibroblasts. Furthermore, a subset of the extracts was assessed for cytotoxicity against primary cultures of human hepatocytes as a model for evaluating potential hepatotoxicity. Roughly one third of cyanobacterial extracts caused cytotoxic effects (i.e. viability < 75%) on human cell lines. Despite the sensitivity differences, high correlation coefficients among the inhibition values were obtained for particular cell systems. This suggests a prevailing general cytotoxic effect of extracts and their constituents. The non-transformed immortalized fibroblasts (Balb/c 3T3) and hepatic cancer line HepG2 exhibited good correlations with primary cultures of human hepatocytes. The presence of cytotoxic fractions in strongly cytotoxic extracts was confirmed by an activity-guided HPLC fractionation, and it was demonstrated that cyanobacterial cytotoxicity is caused by a mixture of components with similar hydrophobic/hydrophilic properties. The data presented here could be used in further research into in vitro testing based on human models for the toxicological monitoring of complex cyanobacterial samples.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1