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Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16

SYSNO ASEP	0464550
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16
Author(s)	Samek, Ota (UPT-D)_{RID, ORCID, SAI} Obruča, S. (CZ) Šiler, Martin (UPT-D)_{RID, ORCID, SAI} Sedláček, P. (CZ) Benešová, P. (CZ) Kučera, D. (CZ) Márová, I. (CZ) Ježek, Jan (UPT-D)_{RID, ORCID, SAI} Bernatová, Silvie (UPT-D)_{RID, SAI} Zemánek, Pavel (UPT-D)_{RID, SAI, ORCID}
Number of authors	10
Source Title	Sensors. - : MDPI Roč. 16, č. 11 (2016), 1808:1-7
Number of pages	7 s.
Publication form	Print - P
Language	eng - English
Country	CH - Switzerland
Keywords	Raman spectroscopy ; Cupriavidus necator H16 ; polyhydroxyalkanoates
Subject RIV	BH - Optics, Masers, Lasers
R&D Projects	GA15-20645S GA ČR - Czech Science Foundation (CSF)
	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UPT-D - RVO:68081731
UT WOS	000389641700041
EID SCOPUS	84994791887
DOI	10.3390/s16111808
Annotation	We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800–1800 cm(−1)) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm(−1)) and for the selection of the two internal standards (DNA at 786 cm(−1)) and Amide I at 1662 cm(−1)). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts—determined by gas chromatography—from 12 percent to 90 percent (w/w) were used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure—from bacteria sampling, centrifugation, and sample preparation to Raman analysis—can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2017

Number of the records: 1