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Chemopreventive agents attenuate rapid inhibition of gap junctional intercellular communication induced by environmental toxicants
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SYSNO ASEP 0464321 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chemopreventive agents attenuate rapid inhibition of gap junctional intercellular communication induced by environmental toxicants Author(s) Babica, Pavel (BU-J) RID, ORCID
Čtveráčková, Lucie (BU-J)
Lenčešová, Zuzana (BU-J)
Trosko, J. E. (US)
Upham, B. L. (US)Number of authors 5 Source Title Nutrition and Cancer-An International Journal - ISSN 0163-5581
Roč. 68, č. 5 (2016), s. 827-837Number of pages 11 s. Language eng - English Country US - United States Keywords gap junctional intercellular communication ; chemopreventive agents ; environmental toxicants Subject RIV FR - Pharmacology ; Medidal Chemistry R&D Projects LH12034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BU-J - RVO:67985939 UT WOS 000380883200013 EID SCOPUS 84976330178 DOI 10.1080/01635581.2016.1180409 Annotation Altered gap junctional intercellular communication (GJIC) has been associated with chemical carcinogenesis, where both chemical tumor promoters and chemopreventive agents (CPAs) are known to conversely modulate GJIC. The aim of this study was to investigate whether attenuation of chemically inhibited GJIC represents a common outcome induced by different CPAs, which could be effectively evaluated using in vitro methods. Rat liver epithelial cells WB-F344 were pretreated with a CPA for either 30min or 24h, and then exposed to GJIC-inhibiting concentration of a selected tumor promoter or environmental toxicant [12-O-tetradecanoylphorbol-13-acetate (TPA), lindane, fluoranthene, 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT), perfluorooctanoic acid (PFOA), or pentachlorophenol]. Out of nine CPAs tested, quercetin and silibinin elicited the most pronounced effects, preventing the dysregulation of GJIC by all the GJIC inhibitors, but DDT. Metformin and curcumin attenuated the effects of three GJIC inhibitors, whereas the other CPAs prevented the effects of two (diallyl sulfide, emodin) or one (indole-3-carbinol, thymoquinone) GJIC inhibitor. Significant attenuation of chemically induced inhibition of GJIC was observed in 27 (50%) out of 54 possible combinations of nine CPAs and six GJIC inhibitors. Our data demonstrate that in vitro evaluation of GJIC can be used as an effective screening tool for identification of chemicals with potential chemopreventive activity. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2017
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