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Protein interactions with quaternized chitosan/heparin multilayers
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SYSNO ASEP 0463595 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Protein interactions with quaternized chitosan/heparin multilayers Author(s) Kumorek, Marta M. (UMCH-V) RID
Kubies, Dana (UMCH-V) RID, ORCID
Riedel, Tomáš (UMCH-V) RID, ORCIDSource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 65, Suppl. 2 (2016), S253-S261Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords heparin ; chitosan ; protein Subject RIV EB - Genetics ; Molecular Biology R&D Projects LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMCH-V - RVO:61389013 UT WOS 000386686800011 EID SCOPUS 84997078065 Annotation Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparinterminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2- presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2017 Electronic address http://www.biomed.cas.cz/physiolres/pdf/65%20Suppl%202/65_S253.pdf
Number of the records: 1