Number of the records: 1

Multienzyme degradation of host serum albumin in ticks

SYSNO ASEP	0463315
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Multienzyme degradation of host serum albumin in ticks
Author(s)	Sojka, Daniel (BC-A)_{RID, ORCID} Pytelková, Jana (UOCHB-X)_RID Perner, Jan (BC-A)_{RID, ORCID} Horn, Martin (UOCHB-X)_{RID, ORCID} Konvičková, Jitka (BC-A) Schrenková, Jana (BC-A) Mareš, Michael (UOCHB-X)_{RID, ORCID} Kopáček, Petr (BC-A)_{RID, ORCID}
Source Title	Ticks and Tick-borne Diseases - ISSN 1877-959X Roč. 7, č. 4 (2016), s. 604-613
Number of pages	10 s.
Publication form	Print - P
Language	eng - English
Country	DE - Germany
Keywords	albumin digestion ; tick ; proteolysis ; gut
Subject RIV	ED - Physiology
Subject RIV - cooperation	Institute of Organic Chemistry and Biochemistry - Biochemistry
R&D Projects	GA13-11043S GA ČR - Czech Science Foundation (CSF)
	GA14-33693S GA ČR - Czech Science Foundation (CSF)
	LO1302 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BC-A - RVO:60077344 ; UOCHB-X - RVO:61388963
UT WOS	000379279000008
EID SCOPUS	84951806339
DOI	10.1016/j.ttbdis.2015.12.014
Annotation	Host blood proteins, represented mainly by hemoglobin and serum albumin, serve as the ultimate source of amino acids needed for de novo protein synthesis during tick development and reproduction. While uptake and processing of hemoglobin by tick gut cells have been studied in detail, molecular mechanisms of host serum albumin degradation remain unknown. In this work, we have used artificial membrane feeding of Ixodes ricinus females on a hemoglobin-free diet in order to characterize the proteolytic machinery involved in albuminolysis. Morphological comparisons of ticks fed on whole blood (BF) and serum (SF) at microscopic and ultrastructural levels showed that albumin and hemoglobin have different trafficking routes in tick gut cells. Analysis in vitro with selective inhibitors demonstrated that albumin is degraded at an acidic pH by a network of cysteine and aspartic peptidases with predominant involvement of cysteine cathepsins having endo- and exopeptidase activities. The cleavage map of albumin and the roles of individual peptidases in albumin degradation were determined. These results indicate that the albuminolytic pathway is controlled by the same proteolytic system that is responsible for hemoglobinolysis. This was further supported by the overall similarity of gut peptidase profiles in SF and BF ticks at the transcriptional and enzymatic activity levels. In conclusion, our work provides evidence that although hemoglobin and albumin are transported differentially during heterophagy they are digested by a common multienzyme proteolytic network. This central digestive system, critical for successful blood feeding in tick females, thus represents a valuable target for novel anti-tick interventions.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2017

Number of the records: 1