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New copolymer brushes for label-free affinity biosensors
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SYSNO ASEP 0462552 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title New copolymer brushes for label-free affinity biosensors Author(s) Brynda, Eduard (UMCH-V) RID
Surman, František (UMCH-V)
Rodriguez-Emmenegger, Cesar (UMCH-V) RID
Riedel, Tomáš (UMCH-V) RID, ORCID
Vaisocherová-Lísalová, Hana (URE-Y)Source Title IEEE SENSORS 2015. - New York : IEEE, 2015 - ISSN 1930-0395 - ISBN 978-1-4799-8203-5 Pages s. 1626-1629 Number of pages 4 s. Publication form Print - P Action IEEE SENSORS 2015 Event date 01.11.2015 - 04.11.2015 VEvent location Busan Country KR - Korea, Republic of Event type WRD Language eng - English Country US - United States Keywords SPR affinity biosensors ; antifouling polymer brushes ; label free detection in blood serum and food extracts Subject RIV BO - Biophysics Subject RIV - cooperation Institute of Radio Engineering and Electronics - Optics, Masers, Lasers R&D Projects GBP205/12/G118 GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 ; URE-Y - RVO:67985882 UT WOS 000380440800424 EID SCOPUS 84963613073 DOI 10.1109/ICSENS.2015.7370596 Annotation Brushes of poly(HPMA-co-CBMAA) copolymer with an adjustable content of functional carboxybetaine (CBMAA) monomer units were grafted from surface of SPR chips using surface initiated atom transfer radical polymerization (SI ATRP). Compared to brushes of of poly(carboxybetaine) (polyCBAA), which so far have provided the best antifouling biorecognition layers, the new brushes were more resistant to undiluted human blood plasma, milk, and crude food extracts, particularly after the covalent attachment of bioreceptors. The incorporation of a selected number of functional CBMAA units in poly(hydroxypropyl methacrylamide) (poly(HPMA)) allowed us to reach ultralow fouling properties better than that of polyCBAA. A typical food-born pathogen bacteria, E.coli O157:H7, was detected in crude extracts of hamburger and lettuce using SPR biosensor coated by biorecognition layers composed of poly(HPMA-co-CBMAA) brushes with covalently attached anti-E. coli antibodies. The SPR biosensor with attached surface antigen of hepatitis B virus HBsAg was used to detect the presence of anti-HBsAg antibodies as biomarkers of hepatitis B infection in standard clinical samples of sera taken from several individuals. The new ultralow fouling biorecognition layers open a feasible way to label-free biosensors capable of in real time detecting analytes in real samples of complex biological media tested for medical diagnostics or food control. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2017
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