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Biocompatible Size-Defined Dendrimer-Albumin Binding Protein Hybrid Materials as a Versatile Platform for Biomedical Applications

SYSNO ASEP	0462486
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Biocompatible Size-Defined Dendrimer-Albumin Binding Protein Hybrid Materials as a Versatile Platform for Biomedical Applications
Author(s)	Malý, J. (CZ) Staněk, Ondřej (MBU-M)_{RID, ORCID} Frolík, J. (CZ) Malý, M. (CZ) Ennen, F. (DE) Appelhans, D. (DE) Semerádtová, A. (CZ) Wróbel, D. (CZ) Stofik, M. (CZ) Knapová, T. (CZ) Kuchař, Milan (BTO-N)_RID Červenková Šťastná, Lucie (UCHP-M)_{RID, ORCID, SAI} Čermák, Jan (UCHP-M)_{RID, ORCID, SAI} Šebo, Peter (MBU-M)_{RID, ORCID} Malý, Petr (BTO-N)_{RID, ORCID}
Source Title	Macromolecular Bioscience. - : Wiley - ISSN 1616-5187 Roč. 16, č. 4 (2016), s. 553-566
Number of pages	14 s.
Language	eng - English
Country	DE - Germany
Keywords	binding domain proteins ; biohybrid structures ; HSA
Subject RIV	CE - Biochemistry
Subject RIV - cooperation	Institute of Biotechnology - Genetics ; Molecular Biology
R&D Projects	GA15-05903S GA ČR - Czech Science Foundation (CSF)
	QJ1210041 GA MZe - Ministry of Agriculture (MZe)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 ; UCHP-M - RVO:67985858
UT WOS	000374262100008
EID SCOPUS	84970897350
DOI	10.1002/mabi.201500332
Annotation	For the design of a biohybrid structure as a ligand-tailored drug delivery system (DDS), it is highly sophisticated to fabricate a DDS based on smoothly controllable conjugation steps. This article reports on the synthesis and the characterization of biohybrid conjugates based on noncovalent conjugation between a multivalent biotinylated and PEGylated poly(amido amine) (PAMAM) dendrimer and a tetrameric streptavidin-small protein binding scaffold. This protein binding scaffold (SA-ABDwt) possesses nM affinity toward human serum albumin (HSA). Thus, well-defined biohybrid structures, finalized by binding of one or two HSA molecules, are available at each conjugation step in a controlled molar ratio.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1