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Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells
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SYSNO ASEP 0462340 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells Author(s) Vašicová, Pavla (MBU-M) RID
Rinnerthaler, M. (AT)
Hašková, Danuša (MBU-M)
Nováková, Lenka (MBU-M)
Malcová, Ivana (MBU-M) RID, ORCID
Breitenbach, M. (AT)
Hašek, Jiří (MBU-M) RID, ORCIDSource Title Microbial cell. - : Shared Science Publishers
Roč. 3, č. 5 (2016), s. 206-214Number of pages 9 s. Language eng - English Country AT - Austria Keywords Abp140-GFP ; Abp1-RFP ; Actin cables Subject RIV EE - Microbiology, Virology R&D Projects GAP305/12/0480 GA ČR - Czech Science Foundation (CSF) GA16-05497S GA ČR - Czech Science Foundation (CSF) 7AMB16AT006 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000377467500004 DOI 10.15698/mic2016.05.499 Annotation Actin filaments form cortical patches and emanating cables in fermenting cells of Saccharomyces cerevisiae. This pattern has been shown to be depolarized in glucose-depleted cells after formaldehyde fixation and staining with rhodamine-tagged phalloidin. Loss of actin cables in mother cells was remarkable. Here we extend our knowledge on actin in live glucosedepleted cells co-expressing the marker of actin patches (Abp1-RFP) with the marker of actin cables (Abp140-GFP). Glucose depletion resulted in appearance of actin patches also in mother cells. However, even after 80 min of glucose deprivation these cells showed a clear network of actin cables labeled with Abp140-GFP in contrast to previously published data. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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