Number of the records: 1
Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application
- 1.
SYSNO ASEP 0462186 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application Author(s) Bidmanová, Š. (CZ)
Kotlanova, M. (CZ)
Rataj, Tomáš (UEK-B)
Damborský, J. (CZ)
Trtílek, M. (CZ)
Prokop, Z. (CZ)Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 84, oct (2016), s. 97-105Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords dehydrochlorinase ; environmental monitoring ; field-testing ; haloalkane dehalogenase ; Halogenated pollutant ; optical biosensor Subject RIV EH - Ecology, Behaviour Research Infrastructure CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i. Institutional support RVO:67179843 - RVO:67179843 UT WOS 000377313300013 EID SCOPUS 84951800327 DOI 10.1016/j.bios.2015.12.010 Annotation An advanced optical biosensor was developed based on the enzymatic reaction with halogenated aliphatic hydrocarbons that is accompanied by the fluorescence change of pH indicator. The device is applicable for the detection of halogenated contaminants in water samples with pH ranging from 4 to 10 and temperature ranging from 5 to 60 °C. Main advantages of the developed biosensor are small size (60×30×190 mm3) and portability, which together with short measurement time of 1 min belong to crucial attributes of analytical technique useful for routine environmental monitoring. The biosensor was successfully applied for the detection of several important halogenated pollutants under laboratory conditions, e.g., 1,2-dichloroethane, 1,2,3-trichloropropane and γ-hexachlorocyclohexane, with the limits of detection of 2.7, 1.4 and 12.1 mg L−1, respectively. The continuous monitoring was demonstrated by repetitive injection of halogenated compound into measurement solution. Consequently, field trials under environmental settings were performed. The presence of 1,2-dichloroethane (10 mg L−1) was proved unambiguously on one of three potentially contaminated sites in Czech Republic, and the same contaminant was monitored on contaminated locality in Serbia. Equipped by Global Positioning System, the biosensor was used for creation of a precise map of contamination. Concentrations determined by biosensor and by gas chromatograph coupled with mass spectrometer exhibited the correlation coefficient of 0.92, providing a good confidence for the routine use of the biosensor system in both field screening and monitoring. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2017
Number of the records: 1