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Calcium-Driven Folding of RTX Domain beta-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts

SYSNO ASEP	0462041
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Calcium-Driven Folding of RTX Domain beta-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts
Author(s)	Bumba, Ladislav (MBU-M)_{RID, ORCID} Mašín, Jiří (MBU-M)_{RID, ORCID} Macek, Pavel (MBU-M) Wald, Tomáš (MBU-M)_RID Motlová, Lucia (MBU-M) Bíbová, Ilona (MBU-M)_RID Klímová, Nela (MBU-M)_ORCID Bednárová, Lucie (UOCHB-X)_{RID, ORCID} Veverka, Václav (UOCHB-X)_{RID, ORCID} Kachala, M. (DE) Svergun, D. I. (DE) Bařinka, Cyril (BTO-N)_{RID, ORCID} Šebo, Peter (MBU-M)_{RID, ORCID}
Source Title	Molecular Cell. - : Cell Press - ISSN 1097-2765 Roč. 62, č. 1 (2016), s. 47-62
Number of pages	17 s.
Language	eng - English
Country	US - United States
Keywords	ADENYLATE-CYCLASE TOXIN ; GRAM-NEGATIVE BACTERIA ; BORDETELLA-PERTUSSIS
Subject RIV	CE - Biochemistry
Subject RIV - cooperation	Institute of Biotechnology - Genetics ; Molecular Biology Institute of Organic Chemistry and Biochemistry - Biochemistry
R&D Projects	LK11205 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LO1304 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA15-11851S GA ČR - Czech Science Foundation (CSF)
	GA13-14547S GA ČR - Czech Science Foundation (CSF)
	GAP302/12/0460 GA ČR - Czech Science Foundation (CSF)
	GAP207/11/0717 GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 ; BTO-N - RVO:86652036
UT WOS	000374119600007
EID SCOPUS	84962446659
DOI	10.1016/j.molcel.2016.03.018
Annotation	Calcium-binding RTX proteins are equipped with C-terminal secretion signals and translocate from the Ca2+-depleted cytosol of Gram-negative bacteria directly into the Ca2+-rich external milieu, passing through the "channel-tunnel" ducts of type I secretion systems (T1SSs). Using Bordetella pertussis adenylate cyclase toxin, we solved the structure of an essential C-terminal assembly that caps the RTX domains of RTX family leukotoxins. This is shown to scaffold directional Ca2+-dependent folding of the carboxy-proximal RTX repeat blocks into beta-rolls. The resulting intramolecular Brownian ratchets then prevent backsliding of translocating RTX proteins in the T1SS conduits and thereby accelerate excretion of very large RTX leukotoxins from bacterial cells by a vectorial "push-ratchet" mechanism.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1