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Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids
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SYSNO ASEP 0461882 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids Author(s) Drini, S. (FR)
Criscuolo, A. (FR)
Lechat, P. (FR)
Imamura, H. (BE)
Skalický, Tomáš (BC-A)
Rachidi, N. (FR)
Lukeš, Julius (BC-A) RID, ORCID
Dujardin, J.-C. (BE)
Späth, G.F. (FR)Source Title Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
Roč. 8, č. 6 (2016), s. 1980-1995Number of pages 16 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Leishmania ; heat shock protein ; HSP70 ; evolution ; phylogeny ; synteny ; copy number variation ; gene lossgene loss Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA14-23986S GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000386368200010 EID SCOPUS 85000524338 DOI 10.1093/gbe/evw140 Annotation All eukaryotic genomes encode multiple members of the heat shock protein 70 (HSP70) family, which evolved distinctive structural and functional features in response to specific environmental constraints. Phylogenetic analysis of this protein family thus can inform on genetic and molecular mechanisms that drive species-specific environmental adaptation. Here we use the eukaryotic pathogen Leishmania spp. as a model system to investigate the evolution of the HSP70 protein family in an early-branching eukaryote that is prone to gene amplification and adapts to cytotoxic host environments by stress-induced and chaperone-dependent stage differentiation. Combining phylogenetic and comparative analyses of trypanosomatid genomes, draft genome of Paratrypanosoma and recently published genome sequences of 204 L. donovani field isolates, we gained unique insight into the evolutionary dynamics of the Leishmania HSP70 protein family. We provide evidence for (i) significant evolutionary expansion of this protein family in Leishmania through gene amplification and functional specialization of highly conserved canonical HSP70 members, (ii) evolution of trypanosomatid-specific, non-canonical family members that likely gained ATPase-independent functions, and (iii) loss of one atypical HSP70 member in the Trypanosoma genus. Finally, we reveal considerable copy number variation of canonical cytoplasmic HSP70 in highly related L. donovani field isolates, thus identifying this locus as a potential hot spot of environment-genotype interaction. Our data draw a complex picture of the genetic history of HSP70 in trypanosomatids that is driven by the remarkable plasticity of the Leishmania genome to undergo massive intra-chromosomal gene amplification to compensate for the absence of regulated transcriptional control in these parasites Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2017
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