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Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots
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SYSNO ASEP 0461676 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots Author(s) Jiskrová, E. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Pospíšilová, H. (CZ)
Holubová, K. (CZ)
Karády, M. (CZ)
Galuszka, P. (CZ)
Robert, S. (BE)
Frébort, I. (CZ)Source Title New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 33, č. 5 (2016), s. 735-742Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords SOLID-PHASE EXTRACTION ; MAIZE BETA-GLUCOSIDASE ; ARABIDOPSIS-THALIANA Subject RIV EB - Genetics ; Molecular Biology R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000378026400015 DOI 10.1016/j.nbt.2015.12.010 Annotation The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2017
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