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Principal component analysis of Raman spectroscopy data for determination of biofilm forming bacteria and yeasts
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SYSNO ASEP 0460214 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Principal component analysis of Raman spectroscopy data for determination of biofilm forming bacteria and yeasts Author(s) Šiler, Martin (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Mlynariková, K. (CZ)
Ježek, Jan (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Holá, M. (CZ)
Růžička, F. (CZ)
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 11 Source Title Proceedings of the 15th International Seminar on Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. - Brno : Institute of Scientific Instruments CAS, 2016 / Mika Filip - ISBN 978-80-87441-17-6 Pages s. 66-67 Number of pages 2 s. Publication form Print - P Action International Seminar on Recent Trends in Charged Particle Optics and Surface Physics Instrumentation /15./ Event date 29.05.2016 - 03.06.2016 VEvent location Skalský dvůr Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords electron microscopy ; biofilm ; Raman spectra Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects GA15-20645S GA ČR - Czech Science Foundation (CSF) GA16-12477S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000391254000030 Annotation Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascular
replacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.
A method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. The
presented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2017 Electronic address http://www.trends.isibrno.cz/
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