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Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms

SYSNO ASEP	0460105
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Sensing microorganisms in the gut triggers the immune response in Eisenia andrei earthworms
Author(s)	Dvořák, Jiří (MBU-M)_{RID, ORCID} Roubalová, Radka (MBU-M)_{RID, ORCID} Procházková, Petra (MBU-M)_{RID, ORCID} Rossmann, Pavel (MBU-M) Škanta, František (MBU-M)_RID Bilej, Martin (MBU-M)_{RID, ORCID}
Source Title	Developmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X Roč. 57, APR 2016 (2016), s. 67-74
Number of pages	8 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Earthworm ; Mucosal immunity ; CCF
Subject RIV	EE - Microbiology, Virology
R&D Projects	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	MBU-M - RVO:61388971
UT WOS	000370091300008
EID SCOPUS	84952802000
DOI	10.1016/j.dci.2015.12.001
Annotation	The tube-within-tube body plan of earthworms is appropriate for studying the interactions of microorganisms with the immune system of body cavities such as the digestive tract and coelom. This study aims to describe the immune response on the molecular and cellular level in the coelomic cavity and the gut of the earthworm Eisenia andrei after experimental microbial challenge by administering two bacterial strains (Escherichia coli and Bacillus subtilis) or yeast Saccharomyces cerevisiae to the environment. The changes in mRNA levels of defense molecules (pattern recognition receptor CCF, lysozyme, fetidin/lysenins) in the coelomocytes and gut tissue were determined by quantitative PCR. The immune response at a cellular level was captured in histological sections, and the expression of CCF was localized using in situ hybridization. Coelomocytes respond to the presence of bacteria in the coelomic cavity by increasing the mRNA levels of defense molecules, especially CCF. The immune response in gut tissue is less affected by microbial stimulation because the epithelial cells of gut exhibit basically strong mRNA synthesis of ccf as a defense against the continuous microbial load in the gut lumen.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1