Number of the records: 1

Cadmium toxicity investigated at the physiological and biophysical levels under environmentally relevant conditions using the aquatic model plant Ceratophyllum demersum

SYSNO ASEP	0459976
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cadmium toxicity investigated at the physiological and biophysical levels under environmentally relevant conditions using the aquatic model plant Ceratophyllum demersum
Author(s)	Andresen, Elisa (BC-A)_{RID, ORCID} Kappel, S. (DE) Stärk, H.-J. (DE) Riegger, U. (DE) Borovec, Jakub (BC-A)_RID Mattusch, J. (DE) Heinz, A. (DE) Schmelzer, C.E.H. (DE) Matoušková, Šárka (GLU-S)_{RID, SAI} Dickinson, B. (US) Küpper, Hendrik (BC-A)_{RID, ORCID}
Source Title	New Phytologist - ISSN 0028-646X Roč. 210, č. 4 (2016), s. 1244-1258
Number of pages	15 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	Ceratophyllum demersum ; Environmentally relevant ; Light-harvesting complexes (LHCs) ; Toxic metals
Subject RIV	CE - Biochemistry
OECD category	Biochemistry and molecular biology
Subject RIV - cooperation	Institute of Geology - Geochemistry
Institutional support	BC-A - RVO:60077344 ; GLU-S - RVO:67985831
UT WOS	000379211400012
EID SCOPUS	84956907993
DOI	10.1111/nph.13840
Annotation	Cadmium (Cd) is an important environmental pollutant and is poisonous to most organisms. We aimed to unravel the mechanisms of Cd toxicity in the model water plant Ceratophyllum demersum exposed to low (nM) concentrations of Cd as are present in nature. Experiments were conducted under environmentally relevant conditions, including nature-like light and temperature cycles, and a low biomass to water ratio. We measured chlorophyll (Chl) fluorescence kinetics, oxygen exchange, the concentrations of reactive oxygen species and pigments, metal binding to proteins, and the accumulation of starch and metals. The inhibition threshold concentration for most parameters was 20 nM. Below this concentration, hardly any stress symptoms were observed. The first site of inhibition was photosynthetic light reactions (the maximal quantum yield of photosystem II (PSII) reaction centre measured as Fv/Fm, light-acclimated PSII activity ΦPSII, and total Chl). Trimers of the PSII light-harvesting complexes (LHCIIs) decreased more than LHC monomers and detection of Cd in the monomers suggested replacement of magnesium (Mg) by Cd in the Chl molecules. As a consequence of dysfunctional photosynthesis and energy dissipation, reactive oxygen species (superoxide and hydrogen peroxide) appeared. Cadmium had negative effects on macrophytes at much lower concentrations than reported previously, emphasizing the importance of studies applying environmentally relevant conditions. A chain of inhibition events could be established.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2017

Number of the records: 1