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The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells
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SYSNO ASEP 0459568 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells Author(s) Trošan, Peter (UEM-P)
Javorková, Eliška (UEM-P) RID
Zajícová, Alena (UEM-P) RID
Hájková, Michaela (UEM-P)
Heřmánková, Barbora (UEM-P) RID, ORCID
Kössl, Jan (UEM-P)
Holáň, Vladimír (UEM-P) RIDSource Title Stem Cells and Development. - : Mary Ann Liebert - ISSN 1547-3287
Roč. 7, č. 17 (2016), s. 23156-23169Number of pages 8 s. Language eng - English Country US - United States Keywords mesenchymal stem cells ; corneal-like cells ; insulin-like growth factor-I ; differentiation Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA14-12580S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 UT WOS 000377383700006 EID SCOPUS 84971501039 DOI 10.1089/scd.2016.0030 Annotation Mesenchymal stem cells (MSCs) possess potent immunoregulatory properties and are a source of numerous growth and trophic factors. These properties make them promising candidate for ocular surface regeneration. It was demonstrated that MSCs can express markers of corneal epithelial cells and keratocytes under selected conditions. In previous study, we found that insulin-like growth factor-I (IGF-I) supports the differentiation of limbal stem cells into corneal-like cells. In the present study, we tested differentiation of mouse bone marrow-derived MSCs into corneal epithelial cells using the extract from the cornea and IGF-I. The results show that MSCs cultured in the presence of corneal extract and IGF-I efficiently differentiate into corneal-like cells. The differentiated cells possess characteristics of corneal epithelial cells and keratocytes and at the same time maintain MSC properties. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2017
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