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DNA damage response during mouse oocyte maturation

SYSNO ASEP	0458917
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	DNA damage response during mouse oocyte maturation
Author(s)	Mayer, Alexandra (UZFG-Y)_RID Baran, Vladimír (UZFG-Y) Sakakibara, Y. (JP) Brzáková, Adéla (UZFG-Y) Ferencová, Ivana (UZFG-Y)_ORCID Motlík, Jan (UZFG-Y)_{RID, ORCID} Kitajima, T. (JP) Schultz, R. M. (US) Šolc, Petr (UZFG-Y)_{RID, ORCID}
Source Title	Cell Cycle. - : Taylor & Francis - ISSN 1538-4101 Roč. 15, č. 4 (2016), s. 546-558
Number of pages	13 s.
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	double strand DNA breaks ; DNA damage ; MRE11 ; meiotic maturation ; mouse oocytes
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LH12057 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED2.1.00/03.0124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UZFG-Y - RVO:67985904
UT WOS	000372110000014
EID SCOPUS	84961285243
DOI	10.1080/15384101.2015.1128592
Annotation	Because low levels of DNA double strand breaks (DSBs) appear not to activate the ATM-mediated prophase I checkpoint in full-grown oocytes, there may exist mechanisms to protect chromosome integrity during meiotic maturation. Using live imaging we demonstrate that low levels of DSBs induced by the radiomimetic drug Neocarzinostatin (NCS) increase the incidence of chromosome fragments and lagging chromosomes but do not lead to APC/C activation and anaphase onset delay. The number of DSBs, represented by gamma H2AX foci, significantly decreases between prophase I and metaphase II in both control and NCS-treated oocytes. Transient treatment with NCS increases >2-fold the number of DSBs in prophase I oocytes, but less than 30% of these oocytes enter anaphase with segregation errors. MRE11, but not ATM, is essential to detect DSBs in prophase I and is involved in H2AX phosphorylation during metaphase I. Inhibiting MRE11 by mirin during meiotic maturation results in anaphase bridges and also increases the number of gamma H2AX foci in metaphase II. Compromised DNA integrity in mirin-treated oocytes indicates a role for MRE11 in chromosome integrity during meiotic maturation.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2017

Number of the records: 1