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A quantitative evaluation of ethylene production in the recombinant cyanobacterium Synechocystis sp PCC 6803 harboring the ethylene-forming enzyme by membrane inlet mass spectrometry
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SYSNO ASEP 0458797 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A quantitative evaluation of ethylene production in the recombinant cyanobacterium Synechocystis sp PCC 6803 harboring the ethylene-forming enzyme by membrane inlet mass spectrometry Author(s) Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Knoop, H. (DE)
Steuer, R. (DE)
Jones, P. R. (GB)
Červený, Jan (UEK-B) RID, ORCID, SAI
Trtílek, M. (CZ)Source Title Bioresource Technology. - : Elsevier - ISSN 0960-8524
Roč. 202, feb (2016), s. 142-151Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords biofuels ; cyanobacteria ; photobioreactor ; MIMS ; biotechnology Subject RIV EH - Ecology, Behaviour R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA15-17367S GA ČR - Czech Science Foundation (CSF) Research Infrastructure CzeCOS II - 90061 - Ústav výzkumu globální změny AV ČR, v. v. i. Institutional support RVO:67179843 - RVO:67179843 UT WOS 000367673500019 EID SCOPUS 84949818111 DOI 10.1016/j.biortech.2015.11.062 Annotation The prediction of the world's future energy consumption and global climate change makes it desirable to identify new technologies to replace or augment fossil fuels by environmentally sustainable alternatives. One appealing sustainable energy concept is harvesting solar energy via photosynthesis coupled to conversion of CO2 into chemical feedstock and fuel. In this work, the production of ethylene, the most widely used petrochemical produced exclusively from fossil fuels, in the model cyanobacterium Synechocystis sp. PCC 6803 is studied. A novel instrumentation setup for quantitative monitoring of ethylene production using a combination of flat-panel photobioreactor coupled to a membrane-inlet mass spectrometer is introduced. Carbon partitioning is estimated using a quantitative model of cyanobacterial metabolism. The results show that ethylene is produced under a wide range of light intensities with an optimum at modest irradiances. The results allow production conditions to be optimized in a highly controlled setup. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2017
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