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Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates

SYSNO ASEP	0458681
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates
Author(s)	Güixens-Gallardo, Pedro (UOCHB-X)_{RID, ORCID} Hocek, Michal (UOCHB-X)_{RID, ORCID} Perlíková, Pavla (UOCHB-X)_{RID, ORCID}
Source Title	Bioorganic and Medicinal Chemistry Letters. - : Elsevier - ISSN 0960-894X Roč. 26, č. 2 (2016), s. 288-291
Number of pages	4 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	DNA polymerases ; nucleotide addition ; primer extension ; oligonucleotides ; twisted intercalating nucleic acid
Subject RIV	CC - Organic Chemistry
R&D Projects	GBP206/12/G151 GA ČR - Czech Science Foundation (CSF)
Institutional support	UOCHB-X - RVO:61388963
UT WOS	000367329700009
EID SCOPUS	84952861737
DOI	10.1016/j.bmcl.2015.12.034
Annotation	A simple and elegant method for inhibition of non-templated nucleotide addition by DNA polymerases and for following DNA 30-heterogeneity in enzymatic DNA synthesis by primer extension (PEX) is described. When template bearing ortho-twisted intercalating nucleic acid (ortho-TINA) at the 5'-end is used, non-templated nucleotide addition is reduced in both the A-and B-family DNA polymerases (KOD XL, KOD (exo-), Bst 2.0, Therminator, Deep Vent (exo-) and Taq). Formation of a single oligonucleotide product was observed with ortho-TINA modified template and KOD XL, KOD (exo-), Bst 2.0, Deep Vent (exo-) and Taq DNA polymerases. This approach can be applied to the synthesis of both unmodified and base-modified oligonucleotides.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2017

Number of the records: 1