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Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity

SYSNO ASEP	0458566
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity
Author(s)	Zasońska, Beata Anna (UMCH-V)_{RID, ORCID} Líšková, A. (SK) Kuricová, M. (SK) Tulinská, J. (SK) Pop-Georgievski, Ognen (UMCH-V)_{RID, ORCID} Čiampor, F. (SK) Vávra, I. (SK) Dušinská, M. (NO) Ilavská, S. (SK) Horváthová, M. (SK) Horák, Daniel (UMCH-V)_{RID, ORCID}
Source Title	Croatian Medical Journal - ISSN 0353-9504 Roč. 57, č. 2 (2016), s. 165-178
Number of pages	14 s.
Language	eng - English
Country	HR - Croatia
Keywords	core-shell maghemite nanoparticles ; proliferative activity of lymphocytes ; phagocytic activity
Subject RIV	CD - Macromolecular Chemistry
R&D Projects	GC16-01128J GA ČR - Czech Science Foundation (CSF)
Institutional support	UMCH-V - RVO:61389013
UT WOS	000377053400009
EID SCOPUS	84971543394
Annotation	To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2017

Number of the records: 1