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Characterization of SCF-Complex during Bovine Preimplantation Development
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SYSNO ASEP 0458448 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of SCF-Complex during Bovine Preimplantation Development Author(s) Benešová, Veronika (UZFG-Y) RID
Kinterová, Veronika (UZFG-Y) ORCID
Kaňka, Jiří (UZFG-Y) RID, ORCID
Toralová, Tereza (UZFG-Y) ORCIDSource Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 11, č. 1 (2016), e0147096-e0147096Number of pages 18 s. Publication form Online - E Language eng - English Country US - United States Keywords F-box protein ; early development Subject RIV EB - Genetics ; Molecular Biology R&D Projects GP13-24730P GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 UT WOS 000369528600014 EID SCOPUS 84958212556 DOI 10.1371/journal.pone.0147096 Annotation The degradation of maternal proteins is one of the most important events during early development, and it is presumed to be essential for embryonic genome activation (EGA), but the precise mechanism is still not known. It is thought that a large proportion of the degradation of maternal proteins is mediated by the ubiquitin-proteolytic system. In this study we focused on the expression of the Skp1-Cullin1-F-box (SCF) complex, a modular RING-type E3 ubiquitin-ligase, during bovine preimplantation development. The complex consists of three invariable components-Cul1, Skp1, Rbx1 and F-box protein, which determines the substrate specificity. The protein level and mRNA expression of all three invariable members were determined. Cul1 and Skp1 mRNA synthesis was activated at early embryonic stages, at the 4c and early 8c stage, respectively, which suggests that these transcripts are necessary for preparing the embryo for EGA. CUL1 protein level increased from MII to the morula stage, with a significant difference between MII and L8c, and between MII and the morula. The CUL1 protein was localized primarily to nuclei and to a lesser extent to the cytoplasm, with a lower signal in the inner cell mass (ICM) compared to the trophectoderm (TE) at the blastocyst stage. The level of SKP1 protein significantly increased from MII oocytes to 4c embryos, but then significantly decreased again. The localization of the SKP1 protein was analysed throughout the cell and similarly to CUL1 at the blastocyst stage, the staining was less intensive in the ICM. There were no statistical differences in RBX1 protein level and localization. The active SCF-complex, which is determined by the interaction of Cul1 and Skp1, was found throughout the whole embryo during preimplantation development, but there was a difference at the blastocyst stage, which exhibits a much stronger signal in the TE than in the ICM. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2017
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