Number of the records: 1
Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution
- 1.
SYSNO ASEP 0455852 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cubozoan genome illuminates functional diversification of opsins and photoreceptor evolution Author(s) Liegertová, Michaela (UMG-J)
Pergner, Jiří (UMG-J)
Kozmiková, Iryna (UMG-J) RID
Fabian, Peter (UMG-J)
Pombinho, António R. (UMG-J)
Strnad, Hynek (UMG-J) RID
Pačes, Jan (UMG-J) RID, ORCID
Vlček, Čestmír (UMG-J) RID
Bartůněk, Petr (UMG-J) RID
Kozmik, Zbyněk (UMG-J) RIDSource Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 5, Jul 8 (2015)Number of pages 19 s. Language eng - English Country GB - United Kingdom Keywords Cubozoan genome ; opsins ; photoreceptor ; evolution Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP305/10/2141 GA ČR - Czech Science Foundation (CSF) LO1220 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000357577400002 DOI 10.1038/srep11885 Annotation Animals sense light primarily by an opsin-based photopigment present in a photoreceptor cell. Cnidaria are arguably the most basal phylum containing a well-developed visual system. The evolutionary history of opsins in the animal kingdom has not yet been resolved. Here, we study the evolution of animal opsins by genome-wide analysis of the cubozoan jellyfish Tripedalia cystophora, a cnidarian possessing complex lens-containing eyes and minor photoreceptors. A large number of opsin genes with distinct tissue-and stage-specific expression were identified. Our phylogenetic analysis unequivocally classifies cubozoan opsins as a sister group to c-opsins and documents lineage-specific expansion of the opsin gene repertoire in the cubozoan genome. Functional analyses provided evidence for the use of the Gs-cAMP signaling pathway in a small set of cubozoan opsins, indicating the possibility that the majority of other cubozoan opsins signal via distinct pathways. Additionally, these tests uncovered subtle differences among individual opsins, suggesting possible fine-tuning for specific photoreceptor tasks. Based on phylogenetic, expression and biochemical analysis we propose that rapid lineage-and species-specific duplications of the intron-less opsin genes and their subsequent functional diversification promoted evolution of a large repertoire of both visual and extraocular photoreceptors in cubozoans. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2016
Number of the records: 1