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TPX2 Protein of Arabidopsis Activates Aurora Kinase 1, But Not Aurora Kinase 3 In Vitro
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SYSNO ASEP 0454970 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title TPX2 Protein of Arabidopsis Activates Aurora Kinase 1, But Not Aurora Kinase 3 In Vitro Author(s) Tomaštíková, Eva (UEB-Q) RID, ORCID
Demidov, D. (DE)
Jeřábková, Hana (UEB-Q) RID, ORCID
Binarová, Pavla (MBU-M) RID, ORCID
Houben, A. (DE)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Petrovská, Beáta (UEB-Q) RID, ORCIDSource Title Plant Molecular Biology Reporter - ISSN 0735-9640
Roč. 33, č. 6 (2015), s. 1988-1995Number of pages 8 s. Language eng - English Country US - United States Keywords Aurora kinase ; Targeting protein for Xklp2 ; In vitro kinase assay Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA14-28443S GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP501/12/2333 GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 ; MBU-M - RVO:61388971 UT WOS 000367464000031 DOI 10.1007/s11105-015-0890-x Annotation Aurora kinases are involved in various mitotic events, including chromosome segregation and bipolar mitotic spindle assembly. In animals, Aurora A is activated and protected by microtubule-associated protein TPX2. Such role in plants is not known. Here, we have assessed the ability of TPX2 of Arabidopsis to regulate Aurora family members in vitro. AtTPX2 acts as substrate as well as activator of AtAurora1, but not AtAurora3. Truncated version of AtTPX2 lacking the Aurora binding domain is unable to activate the kinases; however, it is still phosphorylated. AtTPX2-induced activation of AtAurora1 results in a dramatically increased phosphorylation level of downstream targets, particularly histone H3. The differences in the activation mechanism of AtAurora1 and 3 point to a specific regulation of both kinases, which may play an important role in cell cycle regulation and signaling cascade transduction in plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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