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Novel X-Linked Genes Revealed by Quantitative Polymerase Chain Reaction in the Green Anole, Anolis carolinensis
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SYSNO ASEP 0454370 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel X-Linked Genes Revealed by Quantitative Polymerase Chain Reaction in the Green Anole, Anolis carolinensis Author(s) Rovatsos, M. (CZ)
Altmanová, M. (CZ)
Johnson Pokorná, Martina (UZFG-Y) ORCID
Kratochvíl, L. (CZ)Source Title G3-Genes, Genomes, Genetics. - : Oxford University Press - ISSN 2160-1836
Roč. 4, č. 11 (2014), s. 2107-2113Number of pages 7 s. Publication form Print - P Language eng - English Country US - United States Keywords gene dosage ; lizard ; qPCR Subject RIV EG - Zoology R&D Projects GAP506/10/0718 GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 UT WOS 000345288700006 EID SCOPUS 84937511903 DOI 10.1534/g3.114.014084 Annotation The green anole, Anolis carolinensis (ACA), is the model reptile for a vast array of biological disciplines. It was the first nonavian reptile to have its genome fully sequenced. During the genome project, the XX/XY system of sex chromosomes homologous to chicken chromosome 15 (GGA15) was revealed, and 106 X-linked genes were identified. We selected 38 genes located on eight scaffolds in ACA and having orthologs located on GGA15, then tested their linkage to ACA X chromosome by using comparative quantitative fluorescent real-time polymerase chain reaction applied to male and female genomic DNA. All tested genes appeared to be X-specific and not present on the Y chromosome. Assuming that all genes located on these scaffolds should be localized to the ACA X chromosome, we more than doubled the number of known X-linked genes in ACA, from 106 to 250. While demonstrating that the gene content of chromosome X in ACA and GGA15 is largely conserved, we nevertheless showed that numerous interchromosomal rearrangements had occurred since the splitting of the chicken and anole evolutionary lineages. The presence of many ACA X-specific genes localized to distinct contigs indicates that the ACA Y chromosome should be highly degenerated, having lost a large amount of its original gene content during evolution. The identification of novel genes linked to the X chromosome and absent on the Y chromosome in the model lizard species contributes to ongoing research as to the evolution of sex determination in reptiles and provides important information for future comparative and functional genomics. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2016
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