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FAITH - Fast Assembly Inhibitor Test for HIV
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SYSNO ASEP 0454191 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title FAITH - Fast Assembly Inhibitor Test for HIV Author(s) Hadravová, Romana (UOCHB-X) RID, ORCID
Rumlová, Michaela (UOCHB-X) RID
Ruml, T. (CZ)Number of authors 3 Source Title Virology. - : Elsevier - ISSN 0042-6822
Roč. 486, Dec (2015), s. 78-87Number of pages 10 s. Language eng - English Country US - United States Keywords retrovirus ; HIV ; assembly ; assay ; inhibitor Subject RIV EE - Microbiology, Virology R&D Projects GA14-15326S GA ČR - Czech Science Foundation (CSF) LO1302 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1304 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UOCHB-X - RVO:61388963 UT WOS 000366229800012 EID SCOPUS 84942783664 DOI 10.1016/j.virol.2015.08.029 Annotation Due to the high number of drug-resistant HIV-1 mutants generated by highly active antiretroviral therapy (HAART), there is continuing demand for new types of inhibitors. Both the assembly of the Gag polyprotein into immature and mature HIV-1 particles are attractive candidates for the blocking of the retroviral life cycle. Currently, no therapeutically-used assembly inhibitor is available. One possible explanation is the lack of a reliable and simple assembly inhibitor screening method. To identify compounds potentially inhibiting the formation of both types of HIV-1 particles, we developed a new fluorescent high-throughput screening assay. This assay is based on the quantification of the assembly efficiency in vitro in a 96-well plate format. The key components of the assay are HIV-1 Gag-derived proteins and a dual-labelled oligonucleotide, which emits fluorescence only when the assembly of retroviral particles is inhibited. The method was validated using three (CAI, BM2, FF74) reported assembly inhibitors. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2016 Electronic address http://www.sciencedirect.com/science/article/pii/S0042682215003864
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