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Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp Strain PCC 6803

SYSNO ASEP	0454042
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Lack of Phosphatidylglycerol Inhibits Chlorophyll Biosynthesis at Multiple Sites and Limits Chlorophyllide Reutilization in Synechocystis sp Strain PCC 6803
Author(s)	Kopečná, Jana (MBU-M)_RID Pilný, Jan (MBU-M)_ORCID Krynická, Vendula (MBU-M)_RID Tomčala, Aleš (BC-A)_RID Kis, M. (HU) Gombos, Z. (HU) Komenda, Josef (MBU-M)_{RID, ORCID} Sobotka, Roman (MBU-M)_{RID, ORCID}
Source Title	Plant Physiology. - : Oxford University Press - ISSN 0032-0889 Roč. 169, č. 2 (2015), s. 1307-1317
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	II REACTION-CENTER ; PHOTOSYSTEM-II ; SP PCC-6803
Subject RIV	CE - Biochemistry
R&D Projects	LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EE2.3.30.0059 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GBP501/12/G055 GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971 ; BC-A - RVO:60077344
UT WOS	000365401000036
DOI	10.1104/pp.15.01150
Annotation	The negatively charged lipid phosphatidylglycerol (PG) constitutes up to 10% of total lipids in photosynthetic membranes, and its deprivation in cyanobacteria is accompanied by chlorophyll (Chl) depletion. Indeed, radioactive labeling of the PG-depleted Delta pgsA mutant of Synechocystis sp. strain PCC 6803, which is not able to synthesize PG, proved the inhibition of Chl biosynthesis caused by restriction on the formation of 5-aminolevulinic acid and protochlorophyllide. Although the mutant accumulated chlorophyllide, the last Chl precursor, we showed that it originated from dephytylation of existing Chl and not from the block in the Chl biosynthesis. The lack of de novo-produced Chl under PG depletion was accompanied by a significantly weakened biosynthesis of both monomeric and trimeric photosystem I (PSI) complexes, although the decrease in cellular content was manifested only for the trimeric form. However, our analysis of DpgsA mutant, which lacked trimeric PSI because of the absence of the PsaL subunit, suggested that the virtual stability of monomeric PSI is a result of disintegration of PSI trimers. Interestingly, the loss of trimeric PSI was accompanied by accumulation of monomeric PSI associated with the newly synthesized CP43 subunit of photosystem II. We conclude that the absence of PG results in the inhibition of Chl biosynthetic pathway, which impairs synthesis of PSI, despite the accumulation of chlorophyllide released from the degraded Chl proteins. Based on the knowledge about the role of PG in prokaryotes, we hypothesize that the synthesis of Chl and PSI complexes are colocated in a membrane microdomain requiring PG for integrity.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2016

Number of the records: 1