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Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems
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SYSNO ASEP 0452625 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems Author(s) Mathis, A. (CH)
Depaquit, J. (FR)
Dvořák, V. (CZ)
Tuten, H. (CH)
Banuls, A.-L. (FR)
Halada, Petr (MBU-M) RID, ORCID
Zapata, S. (EC)
Lehrter, V. (FR)
Hlaváčková, K. (CZ)
Prudhomme, J. (FR)
Volf, P. (CZ)
Sereno, D. (FR)
Kaufmann, Ch. (CH)
Pflueger, V. (CH)
Schaffner, F. (CH)Source Title Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 8, MAY 2015 (2015)Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords Arthropod identification ; Bruker ; Centralized reference database Subject RIV CE - Biochemistry R&D Projects GA15-04329S GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 UT WOS 000354433600001 DOI 10.1186/s13071-015-0878-2 Annotation Identical biomarker mass patterns ('superspectra') were obtained with colony-or field-derived specimens of the same species. In the validation study, high quality spectra (i.e. more than 30 evaluable masses) were obtained with all fresh insects from colonies, and with 55/59 insects deep-frozen (liquid nitrogen/-80 degrees C) for up to 25 years. In contrast, only 36/52 specimens stored in ethanol could be identified. This resulted in an overall sensitivity of 87 % (140/161); specificity was 100 %. Duration of storage impaired data counts in the high mass range, and thus cluster analyses of closely related specimens might reflect their storage conditions rather than phenotypic distinctness. A major drawback of MALDI-TOF MS is the restricted availability of in-house databases and the fact that mass spectrometers from 2 companies (Bruker, Shimadzu) are widely being used. We have analysed fingerprints of phlebotomine sand flies obtained by automatic routine procedure on a Bruker instrument by using our database and the software established on a Shimadzu system. The sensitivity with 312 specimens from 8 sand fly species from laboratory colonies when evaluating only high quality spectra was 98.3 %; the specificity was 100 %. The corresponding diagnostic values with 55 field-collected specimens from 4 species were 94.7 % and 97.4 %, respectively. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2016
Number of the records: 1