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Advances in Wheat Genetics: From Genome to Field
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SYSNO ASEP 0451398 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing Author(s) Kobayashi, A. (JP)
Katagiri, S. (JP)
Karasawa, W. (JP)
Takumi, S. (JP)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Ogihara, Y. (JP)
Handa, H. (JP)Number of authors 24 Source Title Advances in Wheat Genetics: From Genome to Field. - Verlag : Springer, 2015 / Handa H. - ISBN 978-4-431-55674-9 Pages s. 101-107 Number of pages 7 s. Number of copy 2500 Number of pages 445 Publication form Print - P Language eng - English Country JP - Japan Keywords genome sequencing ; wheat ; BAC chromosomes Subject RIV EB - Genetics ; Molecular Biology Institutional support UEB-Q - RVO:61389030 DOI 10.1007/978-4-431-55675-6_11 Annotation For a purpose of better understanding the genome structure of wheat and accelerating the development of DNA markers for gene isolations and breeding, the Japanese research group, as a member of The International Wheat Genome Sequencing Consortium, is now conducting the physical mapping and genomic sequencing of wheat chromosome 6B of ‘Chinese Spring’ (CS). BAC libraries were constructed respectively using the short and long arm-specific DNAs extracted from the flow-sorted chromosome 6BS and 6BL of double ditelosomic 6B lines of CS. With a sequence-based finger printing method and contig assembly, the BAC physical maps for the 6BS and 6BL have been successfully established. For validation and chromosomal landing of the BAC contigs, we have developed a large number of 6B-specific DNA markers using the public resources including available EST databases, and 6B survey sequence. In parallel, three reference maps, a highresolution radiation hybrid map, a gametocidal system-derived deletion map and a genetic linkage map, are also under construction for anchoring the BAC contigs to their specific genomic regions. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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