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Tribolium castaneum defensins are primarily active against Gram-positive bacteria

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    SYSNO ASEP0451350
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleTribolium castaneum defensins are primarily active against Gram-positive bacteria
    Author(s) Tonk, M. (DE)
    Knorr, E. (DE)
    Cabezas-Cruz, A. (FR)
    Valdés, James J. (BC-A) RID
    Kollewe, C. (DE)
    Vilcinskas, A. (DE)
    Source TitleJournal of Invertebrate Pathology. - : Elsevier - ISSN 0022-2011
    Roč. 132, NOV 2015 (2015), s. 208-215
    Number of pages8 s.
    Publication formPrint - P
    Languageeng - English
    CountryUS - United States
    KeywordsAntimicrobial peptides ; Defensin ; Innate immunity ; Insects ; Tribolium castaneum ; Gram-positive bacteria
    Subject RIVEI - Biotechnology ; Bionics
    R&D ProjectsEE2.3.30.0032 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportBC-A - RVO:60077344
    UT WOS000365630200023
    DOI10.1016/j.jip.2015.10.009
    AnnotationThe red flour beetle Tribolium castaneum is a destructive insect pest of stored food and feed products, and a model organism for development, evolutionary biology and immunity. The insect innate immune system includes antimicrobial peptides (AMPs) with a wide spectrum of targets including viruses, bacteria, fungi and parasites. Defensins are an evolutionarily-conserved class of AMPs and a potential new source of antimicrobial agents. In this context, we report the antimicrobial activity, phylogenetic and structural properties of three T. castaneum defensins (Def1, Def2 and Def3) and their relevance in the immunity of T. castaneum against bacterial pathogens. All three recombinant defensins showed bactericidal activity against Micrococcus luteus and Bacillus thuringiensis serovar tolworthi, but only Def1 and Def2 showed a bacteriostatic effect against Staphylococcus epidermidis. None of the defensins showed activity against the Gram-negative bacteria Escherichia coli and Pseudomonas entomophila or against the yeast Saccharomyces cerevisiae. All three defensins were transcriptionally upregulated following a bacterial challenge, suggesting a key role in the immunity of T. castaneum against bacterial pathogens. Phylogenetic analysis showed that defensins from T. castaneum, mealworms, Udo longhorn beetle and houseflies cluster within a well-defined clade of insect defensins. We conclude that T. castaneum defensins are primarily active against Gram-positive bacteria and that other AMPs may play a more prominent role against Gram-negative species.
    WorkplaceBiology Centre (since 2006)
    ContactVáclava Lavičková, lavickova@hbu.cas.cz, Tel.: 387 775 889
    Year of Publishing2016