Number of the records: 1
Selenium supplementation induces mitochondrial biogenesis in trophoblasts
- 1.
SYSNO ASEP 0450924 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Selenium supplementation induces mitochondrial biogenesis in trophoblasts Author(s) Khera, A. (AU)
Dong, L. F. (AU)
Holland, O. (AU)
Vanderlelie, J. (AU)
Pasdar, E.A. (AU)
Neužil, Jiří (BTO-N) RID
Perkins, A.V. (AU)Source Title Placenta. - : Elsevier - ISSN 0143-4004
Roč. 36, č. 8 (2015), s. 363-369Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords Selenium ; Reactive oxygen species ; Mitochondrial biogenesis Subject RIV EB - Genetics ; Molecular Biology Institutional support BTO-N - RVO:86652036 UT WOS 000359875000012 DOI 10.1016/j.placenta.2015.06.010 Annotation Introduction: Placental oxidative stress has been implicated in pregnancy complications and previous work has shown that selenium can protect trophoblast mitochondria from oxidative stress. This report examines mitochondrial function and content in trophoblasts supplemented with selenium. Methods: Swan-71, JEG-3 and BeWo cells and placental tissue were incubated with sodium selenite or selenomethionine. Mitochondrial function was examined in a respirometer. Mitochondrial content was determined using RT-PCR. The levels of the mitochondrial biogenesis markers selenoprotein H, PGC-l alpha and NRF-1 was examined by western blotting. Results: Mitochondrial respiration was significantly enhanced post selenium supplementation in cells and tissues. Selenium supplementation increased mitochondrial content and up-regulated mitochondrial biogenesis mediators in cells. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2016
Number of the records: 1