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Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model
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SYSNO ASEP 0450151 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model Author(s) Školoudík, L. (CZ)
Chrobok, V. (CZ)
Kalfert, D. (CZ)
Kočí, Zuzana (UEM-P)
Syková, Eva (UEM-P) RID
Chumak, Tetyana (UEM-P)
Popelář, Jiří (UEM-P) RID
Syka, Josef (UEM-P) RID
Laco, J. (CZ)
Dědková, J. (CZ)
Dayanithi, Govindan (UEM-P) RID
Filip, S. (CZ)Number of authors 12 Source Title Cell Transplantation. - : Sage - ISSN 0963-6897
Roč. 25, č. 7 (2016), s. 1405-1414Number of pages 28 s. Language eng - English Country US - United States Keywords Human bone marrow ; Human mesenchymal stromal cells (hMSCs) ; Middle ear surgery ; Temporal bone Subject RIV FP - Other Medical Disciplines R&D Projects LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 UT WOS 000384801200017 EID SCOPUS 84978134221 DOI 10.3727/096368915X689730 Annotation Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation (p = 0.00174), as well as a significantly higher volume percentage of new immature bone (p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2017
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