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Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum
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SYSNO ASEP 0448547 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Breaking DNA strands by extreme-ultraviolet laser pulses in vacuum Author(s) Nováková, Eva (FZU-D)
Vyšín, Luděk (FZU-D) RID, ORCID
Burian, Tomáš (FZU-D) RID, ORCID
Juha, Libor (FZU-D) RID, ORCID, SAI
Davídková, Marie (UJF-V) RID, ORCID, SAI
Múčka, V. (CZ)
Čuba, V. (CZ)
Grisham, M. E. (US)
Heinbuch, S. (US)
Rocca, J.J. (US)Source Title Physical Review E. - : American Physical Society - ISSN 1539-3755
Roč. 91, č. 4 (2015), "042718-1"-"042718-8"Number of pages 8 s. Language eng - English Country US - United States Keywords XUV ; DNA damages ; single-strand breaks (SSBs) ; double-strand breaks (DSBs) Subject RIV BO - Biophysics R&D Projects GBP108/12/G108 GA ČR - Czech Science Foundation (CSF) GA13-28721S GA ČR - Czech Science Foundation (CSF) Institutional support FZU-D - RVO:68378271 ; UJF-V - RVO:61389005 UT WOS 000353637500008 EID SCOPUS 84929192771 DOI 10.1103/PhysRevE.91.042718 Annotation Ionizing radiation induces a variety of DNA damages including single-strand breaks (SSBs), double-strand breaks (DSBs), abasic sites, modified sugars, and bases. We have studied the nature of DNA damage induced directly by the pulsed 46.9nm (26.5 eV) radiation provided by an extreme ultraviolet (XUV) capillary-discharge Ne-like Ar laser (CDL). Doses up to 45 kGy were delivered with a repetition rate of 3 Hz. We studied the dependence of the yield of SSBs and DSBs of a simple model of DNA molecule (pBR322) on the CDL pulse fluence. Agarose gel electrophoresis method was used for determination of both SSB and DSB yields. The action cross sections of the single and double-strand breaks of pBR322 plasmid DNA in solid state were determined. We observed an increase in the efficiency of strand-break induction in the supercoiled DNA as a function of laser pulse fluence. Results are compared to those acquired at synchrotron radiation facilities and other sources of XUV and soft x-ray radiation. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2016
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