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Nanotherapeutics shielded with a pH responsive polymeric layer
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SYSNO ASEP 0448519 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanotherapeutics shielded with a pH responsive polymeric layer Author(s) Kostka, Libor (UMCH-V) RID, ORCID
Šubr, Vladimír (UMCH-V) RID, ORCID
Laga, Richard (UMCH-V) RID, ORCID
Chytil, Petr (UMCH-V) RID, ORCID
Ulbrich, Karel (UMCH-V) RID
Seymour, L. W. (GB)
Etrych, Tomáš (UMCH-V) RID, ORCIDSource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 64, Suppl. 1 (2015), S29-S40Number of pages 12 s. Language eng - English Country CZ - Czech Republic Keywords nanotherapeutics ; coating ; pH responsive Subject RIV EB - Genetics ; Molecular Biology R&D Projects EE2.3.30.0029 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP301/12/1254 GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 UT WOS 000365010700005 EID SCOPUS 84952656462 Annotation Efficient intravenous delivery is the greatest single hurdle, with most nanotherapeutics frequently found to be unstable in the harsh conditions of the bloodstream. In the case of nanotherapeutics for gene delivery, viral vectors are often avidly recognized by both the innate and the adaptive immune systems. So, most modern delivery systems have benefited from being coated with hydrophilic polymers. Self-assembling delivery systems can achieve both steric and lateral stabilization following surface coating, endowing them with much improved systemic circulation properties and better access to disseminated targets; similarly, gene delivery viral vectors can be ‘stealthed’ and their physical properties modulated by surface coating. Polymers that start degrading under acidic conditions are increasingly investigated as a pathway to trigger the release of drugs or genes once the carrier reaches a slightly acidic tumor environment or after the carrier has been taken up by cells, resulting in the localization of the polymer in acidic endosomes and lysosomes. Advances in the design of acid-degradable drug and gene delivery systems have been focused and discussed in this article with stress placed on HPMA-based copolymers. We designed a system that is able to “throw away” the polymer coat after successful transport of the vector into a target cell. Initial biological studies were performed and it was demonstrated that this principle is applicable for real adenoviral vectors. It was shown that the transfection ability of coated virus at pH 7.4 is 75 times lower then transfection at pH 5.4. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2016 Electronic address http://www.biomed.cas.cz/physiolres/pdf/64%20Suppl%201/64_S29.pdf
Number of the records: 1