Number of the records: 1
Structure of an immunoactive polysaccharide isolated from Korean mulberry fruit (Morus alba L.)
- 1.
SYSNO ASEP 0448044 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Structure of an immunoactive polysaccharide isolated from Korean mulberry fruit (Morus alba L.) Author(s) Bleha, R. (CZ)
Lee, J. S. (KR)
Capek, P. (SK)
Pohl, Radek (UOCHB-X) RID, ORCID
Kim, H. B. (KR)
Choi, D. J. (KR)
Lee, S. (KR)
Lee, J. (KR)
Jang, S. J. (KR)
Synytsya, A. (CZ)
Park, Y. I. (KR)Number of authors 11 Source Title Proceedings of the 10th International Conference on Polysaccharides-Glycoscience. - Prague : Czech Chemical Society, 2014 / Řápková R. ; Čopíková J. ; Šárka E. - ISBN 978-80-86238-70-8 Pages s. 21-26 Number of pages 6 s. Publication form Print - P Action International Conference on Polysaccharides-Glycoscience /10./ Event date 22.10.2014-24.10.2014 VEvent location Prague Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords pectic polysaccharides Subject RIV CC - Organic Chemistry Institutional support UOCHB-X - RVO:61388963 UT WOS 000358813100005 Annotation A water-soluble polysaccharide (JS-MP-1) was isolated from the water extract of Korean white mulberry fruits (Morus alba L.) by ethanol precipitation and then purified by DEAE-cellulose ion exchange chromatography. Obtained final polysaccharide (1600 KDa) consisted of galactose, arabinose and rhamnose as major neutral sugars. JS-MP-1 also contains galacturonic and glucuronic acids (4:1). Sugar linkage, FTIR and correlation NMR analyses confirmed that it is a rhamnogalacturonan type I (RG I), which contains the alternating sequence of 1,4-alpha-D-GalAp and 1,2-alpha-L-Rhap units in the backbone. Neutral sugar side chains of JS-MP1 were identified as (1 -> 5)-alpha-L-arabinan and arabinogalactan type II (AG II) having the (1 -> 6)-beta-D-galactan core. The arabinan side chains are bound to the backbone at the O-4 position of some alpha-L-Rhap units, while the way of linkage between RG I and AG II chains is unclear. It was demonstrated that JS-MP-1 significantly stimulates murine macrophage RAW 264.7 cells to release chemokines (RANTES and MIP-1 alpha) and proinflammatory cytokines like INF-alpha and IL-6, and induce the iNOS and COX-2 gene expression, which are responsible for the production of NO and prostaglandin PGE2, respectively. These results suggest that JS-MP-1 can act as a potent immunomodulator and these observations may support the applicability of this polysaccharide or the water extracts of mulberry fruit can be used as an immunotherapeutic adjuvant or health beneficial food material. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2016
Number of the records: 1