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Complete mitochondrial genomes and nuclear ribosomal RNA operons of two species of Diplostomum (Platyhelminthes: Trematoda): a molecular resource for taxonomy and molecular epidemiology of important fish pathogens
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SYSNO ASEP 0447676 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Complete mitochondrial genomes and nuclear ribosomal RNA operons of two species of Diplostomum (Platyhelminthes: Trematoda): a molecular resource for taxonomy and molecular epidemiology of important fish pathogens Author(s) Brabec, Jan (BC-A) RID, ORCID
Kostadinova, Aneta (BC-A) RID
Scholz, Tomáš (BC-A) RID, ORCID
Littlewood, D. T. J. (GB)Source Title Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 8, JUN 19 2015 (2015), s. 336Number of pages 11 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords Diplostomum (Platyhelminthes: Trematoda) ; fish pathogens ; mitochondrial genome ; ribosomal RNA ; illumina next-generation sequencing ; phylogeny Subject RIV EG - Zoology R&D Projects EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA15-14198S GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000356668000001 DOI 10.1186/s13071-015-0949-4 Annotation Results: We characterised novel complete mt genomes and nuclear rRNA operons of two closely related species, Diplostomum spathaceum and D. pseudospathaceum. Comparative mt genome assessment revealed that the cox1 gene and its 'barcode' region used for molecular identification are the most conserved regions; instead, nad4 and nad5 genes were identified as most promising molecular diagnostic markers. Using the novel data, we provide the first genome wide estimation of the phylogenetic relationships of the order Diplostomida, one of the two fundamental lineages of the Digenea. Analyses of the mitogenomic data invariably recovered the Diplostomidae as a sister lineage of the order Plagiorchiida rather than as a basal lineage of the Diplostomida as inferred in rDNA phylogenies; this was concordant with the mt gene order of Diplostomum spp. exhibiting closer match to the conserved gene order of the Plagiorchiida. Conclusions: Complete sequences of the mt genome and rRNA operon of two species of Diplostomum provide a valuable resource for novel genetic markers for species delineation and large-scale molecular epidemiology and disease ecology studies based on the most accessible life-cycle stages of eye flukes. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2016
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