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pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes
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SYSNO ASEP 0447122 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve SCOPUS Title pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes Author(s) Hanneken, M. (DE)
Šlais, Karel (UIACH-O) RID, ORCID
König, S. (DE)Number of authors 3 Source Title EuPA Open Proteomics - ISSN 2212-9685
Roč. 8, SEP (2015), s. 36-39Number of pages 4 s. Publication form Online - E Language eng - English Country NL - Netherlands Keywords comparative fluorescence gel ; electrophoresis ; protein grid ; azo dyes Subject RIV CB - Analytical Chemistry, Separation Institutional support UIACH-O - RVO:68081715 EID SCOPUS 84940955179 DOI 10.1016/j.euprot.2015.03.003 Annotation Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the y-direction. However, control of the first dimension, pI (x-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2016 Electronic address http://ac.els-cdn.com/S2212968515000094/1-s2.0-S2212968515000094-main.pdf?_tid=7c92fa40-56e6-11e5-b36a-00000aab0f01&acdnat=1441798543_19612c0d7466780944bc4ae22173da92
Number of the records: 1