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Reductive carboxylation and 2-hydroxyglutarate formation by wild-type IDH2 in breast carcinoma cells

SYSNO ASEP	0446813
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Reductive carboxylation and 2-hydroxyglutarate formation by wild-type IDH2 in breast carcinoma cells
Author(s)	Smolková, Katarína (FGU-C)_{RID, ORCID, SAI} Dvořák, Aleš (FGU-C)_{ORCID, RID, SAI} Zelenka, Jaroslav (FGU-C)_RID Vítek, L. (CZ) Ježek, Petr (FGU-C)_{RID, ORCID}
Source Title	International Journal of Biochemistry and Cell Biology. - : Elsevier - ISSN 1357-2725 Roč. 65, AUG 15 (2015), s. 125-133
Number of pages	9 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	reductive carboxylation ; NADPH-dependent isocitrate ; dehydrogenase IDH2 ; hypoxia ; 2-hydroxyglutarate
Subject RIV	FD - Oncology ; Hematology
R&D Projects	GPP301/12/P381 GA ČR - Czech Science Foundation (CSF)
	LF14001 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	FGU-C - RVO:67985823
UT WOS	000358804400013
EID SCOPUS	84930938063
DOI	10.1016/j.biocel.2015.05.012
Annotation	We quantified CO2–dependent reductive carboxylation glutaminolysis (RCG) and CO2–independent 2HG production in HTB-126 and MDA-MB-231 breast carcinoma cells by measuring 13C incorporation from 1-13C-glutamine into citrate, malate, and 2-hydroxyglutarate (2HG), for which we found a substantial enrichment that ceased upon IDH2 silencing. Relatively high basal 12C-2HG levels existed (5-fold higher vs. non-tumor HTB-125 cells) and 13C-2HG was formed despite the absence of any idh2 and idh1 mutations in HTB-126 cells. Since RCG is enhanced at hypoxia (frequent in solid tumors) and 2HG can be formed without idh1/2 mutations, we suggest 2HG as an analytic marker (in serum, urine, or biopsies) predicting malignancy of breast cancer in all patients
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2016

Number of the records: 1