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Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules

SYSNO ASEP	0446563
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules
Author(s)	Bertóková, A. (SK) Vikartovská, A. (SK) Bučko, M. (SK) Gemeiner, P. (SK) Tkáč, J. (SK) Chorvát, D. (SK) Štefuca, V. (SK) Neděla, Vilém (UPT-D)_{RID, ORCID, SAI}
Number of authors	8
Source Title	Biocatalysis and Biotransformation - ISSN 1024-2422 Roč. 33, č. 2 (2015), s. 111-120
Number of pages	10 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	Gluconobacter oxydans ; natural flavors ; phenylacetic acid ; immobilized whole-cell biocatalyst ; polyelectrolyte complex capsules ; environmental scanning electron microscopy
Subject RIV	JA - Electronics ; Optoelectronics, Electrical Engineering
R&D Projects	GA14-22777S GA ČR - Czech Science Foundation (CSF)
Institutional support	UPT-D - RVO:68081731
UT WOS	000359802000006
EID SCOPUS	84938328523
DOI	10.3109/10242422.2015.1053470
Annotation	A high-performance biocatalyst in the form of encapsulated cells of Gluconobacter oxydans have been developed for production of phenylacetic acid (PAA) as a natural flavor component. Polyelectrolyte complex (PEC) capsules consisting of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl2, and NaCl were used for highly controlled and mild encapsulation of cells. Utilization of encapsulated G. oxydans cells was a significant improvement on existing data on operational stability of cells and cumulative product concentration during biocatalytic production of PAA from 2-phenylethanol. Concerning operational stability, encapsulated cells were active over 12 cycles with a high biotransformation rate, while free cells were inactive after 7 cycles of use. The biocatalytic properties of encapsulated G. oxydans were tested in a bubble column reactor over 7 days with a final cumulative product concentration of 25 g/L. High cell viability (90%) was observed within PEC capsules by confocal laser scanning microscopy, performed before and after repetitive PAA production in the bubble column reactor. The surface microstructure of fully hydrated capsules with and without G. oxydans cells was investigated and compared using an environmental scanning electron microscope.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2016

Number of the records: 1