Number of the records: 1
Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes
- 1.
SYSNO ASEP 0446560 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Activity-Based Protein Profiling of Rhomboid Proteases in Liposomes Author(s) Wolf, E. V. (DE)
Seybold, M. (DE)
Hadravová, Romana (UOCHB-X) RID, ORCID
Stříšovský, Kvido (UOCHB-X) RID, ORCID
Verhelst, S. H. L. (DE)Number of authors 5 Source Title Chembiochem. - : Wiley - ISSN 1439-4227
Roč. 16, č. 11 (2015), s. 1616-1621Number of pages 6 s. Language eng - English Country DE - Germany Keywords activity-based protein profiling ; chemical probes ; inhibitors ; intramembrane proteases ; liposomes Subject RIV CE - Biochemistry R&D Projects LK11206 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1302 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UOCHB-X - RVO:61388963 UT WOS 000358330300012 EID SCOPUS 84937251917 DOI 10.1002/cbic.201500213 Annotation Although activity-based protein profiling (ABPP) has been used to study a variety of enzyme classes, its application to intramembrane proteases is still in its infancy. Intramembrane proteolysis is an important biochemical mechanism for activating proteins residing within the membrane in a dormant state. Rhomboid proteases (intramembrane serine proteases) are embedded in the lipid bilayers of membranes and occur in all phylogenetic domains. The study of purified rhomboid proteases has mainly been performed in detergent micelle environments. Here we report on the reconstitution of rhomboids in liposomes. Using ABPP, we have been able to detect active rhomboids in large and giant unilamellar vesicles. We have found that the inhibitor profiles of rhomboids in micelles and liposomes are similar, thus validating previous inhibitor screenings. Moreover, fluorescence microscopy experiments on the liposomes constitute the first steps towards activity-based imaging of rhomboid proteases in membrane environments. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2016
Number of the records: 1