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Biophysical and electrochemical studies of protein-nucleic acid interactions

SYSNO ASEP	0446270
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Biophysical and electrochemical studies of protein-nucleic acid interactions
Author(s)	Bowater, R. P. (GB) Cobb, A:M. (GB) Pivoňková, Hana (BFU-R)_ORCID Havran, Luděk (BFU-R)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID}
Number of authors	5
Source Title	Monatshefte fur Chemie. - : Springer - ISSN 0026-9247 Roč. 146, č. 5 (2015), s. 723-739
Number of pages	17 s.
Publication form	Print - P
Language	eng - English
Country	AT - Austria
Keywords	ISOTHERMAL TITRATION CALORIMETRY ; OSMIUM-TETROXIDE COMPLEXES ; SURFACE-PLASMON RESONANCE
Subject RIV	BO - Biophysics
R&D Projects	GBP206/12/G151 GA ČR - Czech Science Foundation (CSF)
	GAP301/11/2076 GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000353219700002
DOI	10.1007/s00706-014-1405-4
Annotation	This review is devoted to biophysical and electrochemical methods used for studying protein-nucleic acid (NA) interactions. The importance of NA structure and protein-NA recognition for essential cellular processes, such as replication or transcription, is discussed to provide background for description of a range of biophysical chemistry methods that are applied to study a wide scope of protein-DNA and protein-RNA complexes. These techniques employ different detection principles with specific advantages and limitations and are often combined as mutually complementary approaches to provide a complete description of the interactions. Electrochemical methods have proven to be of great utility in such studies because they provide sensitive measurements and can be combined with other approaches that facilitate the protein-NA interactions. Recent applications of electrochemical methods in studies of protein-NA interactions are discussed in detail.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2016

Number of the records: 1