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Instability restricts signaling of multiple fibroblast growth factors
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SYSNO ASEP 0445257 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Instability restricts signaling of multiple fibroblast growth factors Author(s) Buchtová, Marcela (UZFG-Y) RID, ORCID
Chaloupková, R. (CZ)
Zakrzewska, M. (PL)
Veselá, I. (CZ)
Celá, Petra (UZFG-Y) RID
Barathová, J. (CZ)
Gudernová, I. (CZ)
Zajíčková, R. (CZ)
Trantírek, L. (CZ)
Martin, J. (US)
Kostas, M. (PL)
Otlewski, J. (PL)
Damborský, J. (CZ)
Kozubík, Alois (BFU-R) RID, ORCID
Wiedlocha, A. (NO)
Krejčí, P. (CZ)Source Title Cellular and Molecular Life Sciences - ISSN 1420-682X
Roč. 72, č. 12 (2015), s. 2445-2459Number of pages 15 s. Publication form Print - P Language eng - English Country CH - Switzerland Keywords fibroblast growth factor ; FGF ; unstable Subject RIV EA - Cell Biology R&D Projects GA14-31540S GA ČR - Czech Science Foundation (CSF) GBP302/12/G157 GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 ; BFU-R - RVO:68081707 UT WOS 000354883500014 EID SCOPUS 84929710353 DOI 10.1007/s00018-015-1856-8 Annotation Fibroblast growth factors (FGFs) deliver extracellular signals that govern many developmental and regenerative processes, but the mechanisms regulating FGF signaling remain incompletely understood. Here, we explored the relationship between intrinsic stability of FGF proteins and their biological activity for all 18 members of the FGF family. We report that FGF1, FGF3, FGF4, FGF6, FGF8, FGF9, FGF10, FGF16, FGF17, FGF18, FGF20, and FGF22 exist as unstable proteins, which are rapidly degraded in cell cultivation media. Biological activity of FGF1, FGF3, FGF4, FGF6, FGF8, FGF10, FGF16, FGF17, and FGF20 is limited by their instability, manifesting as failure to activate FGF receptor signal transduction over long periods of time, and influence specific cell behavior in vitro and in vivo. Stabilization via exogenous heparin binding, introduction of stabilizing mutations or lowering the cell cultivation temperature rescues signaling of unstable FGFs. Thus, the intrinsic ligand instability is an important elementary level of regulation in the FGF signaling system. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2016
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