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Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR

SYSNO ASEP	0444691
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR
Author(s)	Horák, Daniel (UMCH-V)_{RID, ORCID} Hlídková, Helena (UMCH-V)_{RID, ORCID} Trachtová, Š. (CZ) Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Rittich, B. (CZ) Španová, A. (CZ)
Source Title	European Polymer Journal. - : Elsevier - ISSN 0014-3057 Roč. 68, July (2015), s. 687-696
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	magnetic microspheres ; poly(ethylene glycol) ; real-time PCR
Subject RIV	CD - Macromolecular Chemistry
R&D Projects	GAP206/12/0381 GA ČR - Czech Science Foundation (CSF)
	LH14318 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMCH-V - RVO:61389013
UT WOS	000357230800062
DOI	10.1016/j.eurpolymj.2015.03.036
Annotation	New monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres were synthesized, coated with poly(ethylene glycol) and thoroughly characterized using several methods, such as scanning and transmission electron microscopy equipped with energy-dispersive X-ray spectroscopy (EDX), elemental analysis, ATR FT-IR and atomic absorption spectroscopy. The effect of different coatings of the microspheres on the DNA amplification was finally investigated using a real-time polymerase chain reaction (qPCR). The particles with a relatively high density of poly(ethylene glycol) and a low amount of carboxyl groups on the surface appeared suitable for DNA isolation.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2016

Number of the records: 1