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Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding

SYSNO ASEP	0444555
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding
Author(s)	Hasan, Shakir (MBU-M) Osičková, Adriana (MBU-M)_{RID, ORCID} Bumba, Ladislav (MBU-M)_{RID, ORCID} Novák, Petr (MBU-M)_{RID, ORCID} Šebo, Peter (MBU-M)_{RID, ORCID} Osička, Radim (MBU-M)_{RID, ORCID}
Source Title	FEBS Letters. - : Wiley - ISSN 0014-5793 Roč. 589, č. 3 (2015), s. 374-379
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Adenylate cyclase toxin ; CD11b/CD18 ; Complement receptor type 3
Subject RIV	CE - Biochemistry
R&D Projects	GAP302/11/0580 GA ČR - Czech Science Foundation (CSF)
	GA15-09157S GA ČR - Czech Science Foundation (CSF)
	GA15-11851S GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000349403300013
DOI	10.1016/j.febslet.2014.12.023
Annotation	The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of CR3 were substituted with glutamine residues that cannot be glycosylated. Examination of CR3 mutant variants and mass spectrometry analysis of the N-glycosylation pattern of CR3 revealed that N-glycans located in the C-terminal part of the CD11b subunit are involved in binding and cytotoxic activity of CyaA. We suggest that these N-glycans form a defined clustered saccharide patch that enables multivalent contact of CR3 with CyaA, enhancing both affinity and specificity of the integrin-toxin interaction.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2016

Number of the records: 1