Number of the records: 1
Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig
- 1.
SYSNO ASEP 0443826 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig Author(s) Plánská, Daniela (UZFG-Y)
Burocziová, Monika (MBU-M)
Strnádel, Ján (UZFG-Y) RID
Horák, Vratislav (UZFG-Y) RID, ORCIDSource Title Acta histochemica et cytochemica - ISSN 0044-5991
Roč. 48, č. 1 (2015), s. 15-26Number of pages 12 s. Publication form Print - P Language eng - English Country DE - Germany Keywords collagen IV ; laminin ; MeLiM ; porcine melanoma ; spontaneous regression Subject RIV EB - Genetics ; Molecular Biology R&D Projects ED2.1.00/03.0124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UZFG-Y - RVO:67985904 ; MBU-M - RVO:61388971 UT WOS 000354928000003 EID SCOPUS 84923648899 DOI 10.1267/ahc.14020 Annotation Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2016
Number of the records: 1