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FRAP & FLIP: Two Sides of the Same Coin?
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SYSNO ASEP 0443270 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title FRAP & FLIP: Two Sides of the Same Coin? Author(s) Papáček, Š. (CZ)
Jablonský, J. (CZ)
Matonoha, Ctirad (UIVT-O) RID, SAI
Kaňa, Radek (MBU-M) RID, ORCID
Kindermann, S. (AT)Source Title Bioinformatics and Biomedical Engineering, Part II, 2. - Cham : Springer, 2015 / Ortuno F. ; Rojas I. - ISSN 0302-9743 - ISBN 978-3-319-16479-3 Pages s. 444-455 Number of pages 12 s. Publication form Print - P Action IWBBIO 2015. International Conference /3./ Event date 15.04.2015-17.04.2015 VEvent location Granada Country ES - Spain Event type WRD Language eng - English Country CH - Switzerland Keywords FRAP ; FLIP ; sensitivity analysis ; parameter identification Subject RIV BA - General Mathematics Subject RIV - cooperation Institute of Microbiology - General Mathematics R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP501/12/0304 GA ČR - Czech Science Foundation (CSF) Institutional support UIVT-O - RVO:67985807 ; MBU-M - RVO:61388971 UT WOS 000410784400044 EID SCOPUS 84925238673 DOI 10.1007/978-3-319-16480-9_44 Annotation The aim of this study is to point out the similarity and differences of data processing based on either FRAP (Fluorescence Recovery After Photobleaching) or FLIP (Fluorescence Loss In Photobleaching) experimental techniques. The core idea, closely related to the sensitivity analysis, is based on discerning between relevant and irrelevant data. Presented mathematical model allows to visualize the mutual relation between the FRAP and FLIP methods. The whole concept resides in the processing of full spatio-temporal data instead of the space averaged time series (FRAP recovery curves). The method theoretically confirms the empirical knowledge, that the mobility of fluorescent molecules can be determined with both FRAP and FLIP methods (using the full data approach). Our analysis, based on the idealized theoretical case study, supports the conclusions of our recent experiments and thus it validates the reliability of our new approach. The presented finding are expected to be reflected into experimental protocol setup as well as we will continue working on the further enhancing the method of parameter identification. Workplace Institute of Computer Science Contact Tereza Šírová, sirova@cs.cas.cz, Tel.: 266 053 800 Year of Publishing 2016
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