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Laser applications for high-sensitive detection of biologically important molecules
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SYSNO ASEP 0441039 Document Type D - Thesis R&D Document Type The record was not marked in the RIV Title Laser applications for high-sensitive detection of biologically important molecules Author(s) Přikryl, Jan (UIACH-O) RID, ORCID Number of authors 1 Issue data Brno: Masarykova univerzita, 2015 Number of pages 105 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords luminescence ; surface-enhanced Raman spectrometry ; 3D print Subject RIV CB - Analytical Chemistry, Separation Institutional support UIACH-O - RVO:68081715 Annotation High-sensitive methods allowing detection and identification of biologically important analytes are currently requested for the majority of applications in biology, medicine and related branches of science. The remarkable progress of new spectroscopic methods enabled especially by improvements in construction of light detectors and light sources in the last three decades. The presented work submits an overview of highly sensitive spectroscopic methods with emphasis on surface-enhanced Raman spectrometry and various luminescence methods. The analytical applications are presented as detection methods of capillary electrophoresis, imaging methods and single cell analysis. The optimization of surface enhanced Raman spectrometry, laser or LED induced fluorescence, time-resolved photoluminescence, total-internal reflection photoluminescence and chemiluminescence for high sensitivity and selectivity bioanalyses is demonstrated. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2015
Number of the records: 1