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Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B-Structuromics
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SYSNO ASEP 0440773 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B-Structuromics Author(s) Falk, Martin (BFU-R) RID, ORCID
Hausmann, M. (DE)
Lukášová, Emilie (BFU-R) RID, ORCID
Biswas, A. (DE)
Hildenbrand, G. (DE)
Davídková, Marie (UJF-V) RID, ORCID, SAI
Krasavin, E. (RU)
Kleibl, Z. (CZ)
Falková, Iva (BFU-R) ORCID
Ježková, L. (CZ)
Štefančíková, Lenka (BFU-R)
Ševčík, J. (CZ)
Hofer, Michal (BFU-R) RID, ORCID
Bačíková, Alena (BFU-R)
Matula, Pavel (BFU-R) RID
Boreyko, A. (RU)
Vachelová, Jana (UJF-V) RID, SAI
Jelínek Michaelidesová, Anna (UJF-V) RID, ORCID, SAI
Kozubek, Stanislav (BFU-R) RIDNumber of authors 19 Source Title Critical Reviews in Eukaryotic Gene Expression - ISSN 1045-4403
Roč. 24, č. 3 (2014), s. 225-247Number of pages 23 s. Publication form Print - P Language eng - English Country US - United States Keywords omics ; ionizing radiation ; low-dose dilemma ; biological complexity and variability ; higher-order chromatin structure ; DNA damage response ; formation of chromosomal translocations ; confocal microscopy ; localization nanoscopy Subject RIV BO - Biophysics Subject RIV - cooperation Institute of Biophysics - Biophysics R&D Projects GBP302/12/G157 GA ČR - Czech Science Foundation (CSF) GAP302/10/1022 GA ČR - Czech Science Foundation (CSF) LD12039 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LD12008 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2011019 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 ; UJF-V - RVO:61389005 UT WOS 000339087800004 EID SCOPUS 84902656014 DOI 10.1615/CritRevEukaryotGeneExpr.2014010313 Annotation n this Part B we show how high-resolution confocal microscopy as well as novel approaches of molecular localization nanoscopy fill the gaps to successfully place Omics data in the context of space and time. The dynamics of double-strand breaks during repair processes and chromosomal rearrangements at the microscale correlated to aberration induction are explained. For the first time we visualize pan-nuclear nucleosomal rearrangements and clustering at the nanoscale during repair processes. Finally, we introduce a novel method of specific chromatin nanotargeting based on a computer database search of uniquely binding oligonucleotide combinations (COMBO-FISH). With these challenging techniques on hand, we speculate future perspectives that may combine specific COMBO-FISH nanoprobing and structural nanoscopy to observe structure-function correlations in living cells in real-time. Thus, the Omics networks obtained from function analyses may be enriched by real-time visualization of Structuromics. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2015
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