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Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

SYSNO ASEP	0440405
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes
Author(s)	Nevrtalová, Eva (BFU-R) Baloun, Jiří (BFU-R) Hudzieczek, Vojtěch (BFU-R)_ORCID Čegan, Radim (BFU-R)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Šafář, Jan (UEB-Q)_{RID, ORCID} Milde, D. (CZ) Hobza, Roman (BFU-R)_{RID, ORCID}
Number of authors	9
Source Title	Protoplasma - ISSN 0033-183X Roč. 251, č. 6 (2014), s. 1427-1439
Number of pages	13 s.
Publication form	Print - P
Language	eng - English
Country	AT - Austria
Keywords	Copper ; Gene duplication ; Metallothionein
Subject RIV	BO - Biophysics
Subject RIV - cooperation	Institute of Experimental Botany - Botanics
R&D Projects	GAP501/12/2220 GA ČR - Czech Science Foundation (CSF)
	GBP501/12/G090 GA ČR - Czech Science Foundation (CSF)
	GP13-34962P GA ČR - Czech Science Foundation (CSF)
	GA522/09/0083 GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000344336500013
DOI	10.1007/s00709-014-0644-x
Annotation	Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1