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Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes

SYSNO ASEP	0440398
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes
Author(s)	Baloun, Jiří (BFU-R) Nevrtalová, Eva (BFU-R) Kováčová, Viera (BFU-R) Hudzieczek, Vojtěch (BFU-R)_ORCID Čegan, Radim (BFU-R)_{RID, ORCID} Vyskot, Boris (BFU-R)_{RID, ORCID} Hobza, Roman (BFU-R)_{RID, ORCID}
Number of authors	7
Source Title	Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617 Roč. 171, č. 13 (2014), s. 1188-1196
Number of pages	9 s.
Publication form	Print - P
Language	eng - English
Country	PT - Portugal
Keywords	Copper ; Genes coding ROS-eliminating and Cu-transporting proteins ; RNA-Seq database
Subject RIV	BO - Biophysics
R&D Projects	GBP501/12/G090 GA ČR - Czech Science Foundation (CSF)
	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000345629900011
DOI	10.1016/j.jplph.2014.04.014
Annotation	Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1