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Plant Cell Morphogenesis: Methods and Protocols
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SYSNO ASEP 0439872 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Image Analysis: Basic Procedures for Description of Plant Structures Author(s) Albrechtová, J. (CZ)
Kubínová, Z. (CZ)
Soukup, A. (CZ)
Janáček, Jiří (FGU-C) RID, ORCIDSource Title Plant Cell Morphogenesis: Methods and Protocols, Vol. 1080. - New York : Humana Press, 2014 / Žárský V. ; Cvrčková F. - ISSN 1064-3745 - ISBN 978-1-62703-643-6 Pages s. 67-76 Number of pages 10 s. Number of pages 285 Publication form Online - E Language eng - English Country US - United States Keywords experiment design ; image analysis ; stereology Subject RIV EA - Cell Biology R&D Projects GAP501/10/0340 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 UT WOS 000326510400006 EID SCOPUS 84887440757 DOI 10.1007/978-1-62703-643-6_5 Annotation This chapter gives examples of basic procedures of quantification of plant structures with the use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified "rules of thumb" are presented. The basic procedures mentioned in the text are (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2015
Number of the records: 1